It depends on your motherboard. This PCI card is keyed for 5V PCI and PCI-X slots. If your motherboard's PCI or PCI-X slots are keyed for 5V, this will work. If it is keyed for 3.3V, it will not. You can tell which your motherboard is keyed for by the location of the notch on the slot. To confirm whether this PCI card will fit into your PCI-X slot, refer to your motherboard's documentation.